A comparison of beer volatiles extraction using HS-SPME and DLLME for analysis by GC-MS

Author Name(s)

Carmen Cortez Ortega

Faculty Advisor(s)

Blake Gillespie

Abstract

Understanding yeast’s contribution to flavor development in beer brewing often requires rapid, reproducible extraction of volatiles from aqueous solution for mass spectral analysis. HS-SPME is a time consuming process in which reproducibility is a challenge. A more efficient process is dispersive liquid-liquid microextraction (DLLME). In comparison to HS-SPME, DLLME allows many samples to be rapidly and simultaneously extracted. Using volatiles commonly found in beers as standards, we are optimizing DLLME extraction procedures and compare our sample recovery with HS-SPME. We describe recovering standard volatiles at concentrations as low as 10 ppm from water, using both techniques. Additionally, we show good recovery of volatiles from beer using DLLME, and present a quantitative comparison of DLLME and HS-SPME.

Presentation

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